A5060
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 | Immunoblotting Whole cell extract of chicken gizzard was separated on SDS-PAGE and blotted with Rabbit Anti-Actin (Cat. No. A5060). The antibody was developed with Goat Anti-Rabbit IgG, Alkaline Phosphatase conjugate (Cat. No. A9919) and NBT/BCIP substrate. Lanes A: Antibody dilution 1:500 B: Negative control (only secondary antibody) |
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A5060
Sigma
Anti-Actin (20-33) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
| MDL number: | MFCD01092261 |
Description
| Immunogen | N-terminal actin peptide (amino acid residues 20-33) attached to a multiple antigen peptide (MAP) backbone. |
| Other Notes | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin. |
| Specificity | The antibody shows a broad reactivity among actin isoforms and across a range of organisms. It specifically stains typical stress fibers in cultured cells using an indirect immunofluorescent labeling technique and can be used as a probe of the N-terminal region of actin. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative. |
Properties
| antibody form | IgG fraction of antiserum |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 42 kDa |
| species reactivity | wide range (animal), invertebrates |
| application(s) | immunoblotting: 1:250 using human and animal tissue extracts |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using human or animal tissue sections | |
| immunohistochemistry (frozen sections): suitable using human or animal tissue sections | |
| indirect immunofluorescence: 1:200 using cultured human fibroblasts | |
| microarray: suitable | |
| Gene Information | human ... ACTA1(58) |
| rat ... Acta1(29437) | |
| mouse ... Acta1(11459) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
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References
| reference | Meiller, A., et al., p53-dependent stimulation of redox-related genes in the lymphoid organs of γ-irradiated mice—identification of Haeme-oxygenase 1 as a direct p53 target gene Nucleic Acids Res. 35, 6924-6934, (2007) Abstract |
| Xia, S., and Tully, T., Segregation of Odor Identity and Intensity during Odor Discrimination in Drosophila Mushroom Body PLoS Biol. 5, e264, (2007) Abstract | |
| Toth, A., et al., Human Keratinocytes Are Vanilloid Resistant PLoS ONE 3, e3419, (2008) Abstract |
